Teachers: Marco Biggiogera, Irene Masiello
The course is aimed at presenting some advanced microscopy techniques, in their application to cell and molecular biology.
Basic concepts will first be given on fluorescence microscopy and on conventional transmission and scanning electron microscopy; students will then be introduced to more refined new tools (such as confocal microscopy and Energy Filtering electron microscopy, EFTEM) for visualizing in situ subcellular structures, also under living conditions. FRET (fluorescence resonance energy transfer), FRAP (fluorescence recovery after photobleaching), FLIP (fluorescence loss in photo bleaching), electron tomography and electron spectroscopic imaging, as well as correlative microscopy will be also considered in their application to different cell models.
Superresolution: scanning tunneling microscopy, atomic force microscopy; Stimulated Emission Depletion (STED), Photoactivated Localization Microscopy (PALM), Stochastic optical reconstruction microscopy (STORM). Some of the above mentioned techniques will be used in the practical part of the course.
Chandler & Roberson. BIOIMAGING. Jones & Bartlett Publishers